Our new preprint about descSPIM was submitted and open in bioRxiv. Despite the easier use of multiple tissue clearing techniques in recent years, poor access to adequate light-sheet fluorescence microscopy remains a major obstacle for biomedical end users. Here, we propose a solution by developing descSPIM (desktop-equipped SPIM for cleared specimens) as a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practically substantial do-it-yourself light-sheet microscopy. Academically open-sourced, descSPIM allows routine three-dimensional imaging of cleared samples in minutes.


Image: modified Figs of the preprint; Kohei Otomo


メールアドレスが公開されることはありません。 が付いている欄は必須項目です

POSTED BY Otomo | 5月, 02, 2023 |